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7. | | ROSA, P. M. da; ANTONIASSI, R.; GONÇALVES, E. B.; BIZZO, H. R.; SILVA, A. J. R. da. Extração de ácido clorogênico de farelo de girassol desengordurado. Ciência Rural, Santa Maria, v. 41, n. 4, p. 719-724, abr. 2011. Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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10. | | ROSA, P. M.; ANTONIASSI, R.; FREITAS, S. C.; BIZZO, H. R.; ZANOTTO, D. L.; OLIVEIRA, M. F. de; CASTIGLIONI, V. B. R. Chemical composition of brazilian sunflower varieties. Helia, Novi Sad, v. 32, n. 50, p. 145-156, 2009. Biblioteca(s): Embrapa Soja. |
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11. | | ROSA, P. M.; ANTONIASSI, R.; FREITAS, S. C.; BIZZO, H. R.; ZANOTTO, D. L.; OLIVEIRA, M. F.; CASTIGLIONI, V. B. R. Chemical composition of brazilian sunflower varieties. Helia, Novi Sad, v. 32, n. 50, p. 145-156, 2009. Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Suínos e Aves. |
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12. | | CHAVES, A. C. S. D.; BRAGANÇA, I. A.; ROSA, P. M.; SÁ, D. de G. C. F. de; GOMES, F. dos S.; CABRAL, L. M. C. Avaliação do efeito corante de polpa de juçara atomizada em sorvete e iogurte de frutas vermelhas. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS E NUTRIÇÃO, 15., 2023, Campinas. A revolução da ciência de alimentos e nutrição: alimentando o mundo de forma sustentável: caderno [eletrônico] de resumos. Campinas: Galoá, 2023. Pôster 164512. SLACAN. Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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13. | | ROSA, P. M. S.; MONTEIRO, C. A. S.; LEAL, G. R.; CAMARGO, A. J. R.; SERAPIÃO, R. V.; LIMA, M. R.; GARCIA, J. M.; OLIVEIRA, C. S. Association of two epigenetic modulators on the initial development of female bovine embryos. Animal Reproduction, v. 15, n. 3, p. 484, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018. Biblioteca(s): Embrapa Gado de Leite. |
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14. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASSOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Caracterização de oócitos de animais pré-púberes da raga Gir: tamnho do oócito, morfologia e atividade metabólica. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. Biblioteca(s): Embrapa Gado de Leite. |
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15. | | DIAS, L. P.; ROSA, P. M. S.; CAMARGO, A. J. R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A.; OLIVEIRA, C. S. Follicular dynamics of gry and Holstein oocyte donors kept under tropical conditions In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2016, Louisville. Reproduction, fertility and development: proceedings. Louisville: [s.n.], 2016. Biblioteca(s): Embrapa Gado de Leite. |
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16. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Hormonal ovulation induction in prepubertal GYR heifers In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tours: Society for Reproductive Biology, 2016. p. 516-517. Biblioteca(s): Embrapa Gado de Leite. |
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17. | | ROSA, P. M. da S.; CAMARGO, A. J. dos R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A.; OLIVEIRA, C. S. Eficiência reprodutiva de novilhas pré-púberes da raça Gir na produção in vitro de embriões. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 2 p. Biblioteca(s): Embrapa Gado de Leite. |
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18. | | DIAS, L. P.; ROSA, P. M. da S.; CAMARGO, A. J. dos R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A.; OLIVEIRA, C. S. Dinâmica folicular em doadoras de oócito das raças Gir e Holandesa mantidas em condições tropicais. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 2 p. Biblioteca(s): Embrapa Gado de Leite. |
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19. | | ROSA, P. M.; CAMPOS, T. de; SOUSA, A. C. B. de; SFORÇA, D. A.; TORRES, G. A. M.; SOUZA, A. P. de. Potato cultivar identification using molecular markers. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 1, p. 110-113, jan. 2010. Notas Científicas.
Título em português: Identificação de cultivares de batata por marcadores moleculares. Biblioteca(s): Embrapa Unidades Centrais. |
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20. | | ROSA, P. M. da S.; CAMARGO, A. J. DOS R.; SERAPIÃO, R, V.; ALVES, B. R. C.; NOGUEIRA, G. DE. P.; OLIVEIRA, C. S. Perfil de secreção de hormônio luteinizante em novilhas pré-púberes da raça Gir, submetidas a protocolo de indução de ovulação. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 20. 2017, 4 p. Anais... Juiz de Fora/MG. Embrapa Gado de Leite. Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 33 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
14/01/2020 |
Data da última atualização: |
06/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
OLIVEIRA, C. S.; BARROS, B. A. F. de; MONTEIRO, C. A. S.; ROSA, P. M. S.; LEAL, G. R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A. |
Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; B. A. F. de Barros; C. A. S. Monteiro; P. M. S. Rosa; G. R. Leal; Raquel Varela Serapião; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
Título: |
Individual assessment of bovine embryo development using a homemade chamber reveals kinetic patterns of success and failure to reach blastocyst stage. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Systems Biology in Reproductive Medicine, v. 65, n. 4, p. 301-311, 2019. |
DOI: |
https://doi.org/10.1080/19396368.2019.1589601 |
Idioma: |
Inglês |
Conteúdo: |
Most early developmental data are lost in bovine embryo culture systems. We developed and validated a method for culture of bovine embryos in groups that allow individual assessment. An autoclavable low-cost multiembryo chamber (MEC) was prepared using a polyester mesh fixed to a glass coverslip. Embryonic development was not affected by MEC. Compared to conventional bovine culture system (oil-covered drops, control), cleavage (C, 71.2 ± 7.8%; MEC, 74.3 ± 6.0%), blastocyst rate (C, 29.9 ± 4.4%; MEC, 28.3 ± 5.0%) and blastocyst cell number (C, 94.1 ± 9.7; MEC, 92.9 ± 5.3) were similar. Caspase 3 positive cell index in blastocysts was increased in MEC group, but apoptosis rate was below 5% (C, 2.9 ± 0.5; MEC, 4.6 ± 0.6). Using MEC, we performed a retrospective analysis for 'failure' and 'success' embryos, based on their ability to reach the blastocyst stage. We detected the majority of 'success' embryos displayed 8 cells at 48 h post-insemination (hpi) (48.7%), but blastocysts derived from this pattern presented lower cell numbers (91.3 ± 4.2 vs. 107.9 ± 4.9) and higher apoptosis index (6.2 ± 0.6 vs. 4.4 ± 0.5) than blastocysts from 4-cell embryos at 48 hpi. Most (72.0%) embryos that were at morula stage 120 hpi reached blastocyst stage at 168 hpi. Those blastocysts presented more number of cells than blastocysts derived from embryos exhibiting 16 cells at 120 hpi (108.6 ± 4.1 vs. 83.9 ± 4.8). Combination of embryo kinetics data at 48 and 120 hpi revealed high chances of blastocyst formation for patterns: 8 cells/morula, 4 cells/morula, 8 cells/16 cells and 4 cells/16 cells. Blastocysts formed from 4-cell/morula and 8-cell/morula patterns represented 69% of all 168 hpi blastocysts. Blastocysts derived from 4 cells/16 cells displayed decreased apoptosis (3.1 ± 0.6). Our results suggest that MEC can be used for bovine embryo culture without detrimental effects on development and can help to predict blastocyst formation and quality of in vitro fertilization (IVF) embryos. Abbreviations: BSA: bovine serum albumine; COC: cumulus-oocyte complex; FERT-TALP: Tyrode's albumin lactate pyruvate fertilization; FBS: fetal bovine serum; IVF: in vitro fertilization; MEC: multiembryo chamber; PBS: phosphate buffered saline; SOF-AA: synthetic oviductal fluid with amino acids medium; TCM: Tissue Culture Medium. MenosMost early developmental data are lost in bovine embryo culture systems. We developed and validated a method for culture of bovine embryos in groups that allow individual assessment. An autoclavable low-cost multiembryo chamber (MEC) was prepared using a polyester mesh fixed to a glass coverslip. Embryonic development was not affected by MEC. Compared to conventional bovine culture system (oil-covered drops, control), cleavage (C, 71.2 ± 7.8%; MEC, 74.3 ± 6.0%), blastocyst rate (C, 29.9 ± 4.4%; MEC, 28.3 ± 5.0%) and blastocyst cell number (C, 94.1 ± 9.7; MEC, 92.9 ± 5.3) were similar. Caspase 3 positive cell index in blastocysts was increased in MEC group, but apoptosis rate was below 5% (C, 2.9 ± 0.5; MEC, 4.6 ± 0.6). Using MEC, we performed a retrospective analysis for 'failure' and 'success' embryos, based on their ability to reach the blastocyst stage. We detected the majority of 'success' embryos displayed 8 cells at 48 h post-insemination (hpi) (48.7%), but blastocysts derived from this pattern presented lower cell numbers (91.3 ± 4.2 vs. 107.9 ± 4.9) and higher apoptosis index (6.2 ± 0.6 vs. 4.4 ± 0.5) than blastocysts from 4-cell embryos at 48 hpi. Most (72.0%) embryos that were at morula stage 120 hpi reached blastocyst stage at 168 hpi. Those blastocysts presented more number of cells than blastocysts derived from embryos exhibiting 16 cells at 120 hpi (108.6 ± 4.1 vs. 83.9 ± 4.8). Combination of embryo kinetics data at 48 and 120 hpi revealed high chances of blast... Mostrar Tudo |
Palavras-Chave: |
Culture system; Embryo kinetics; Embryonic quality; Single embryo culture. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03220naa a2200253 a 4500 001 2118728 005 2024-02-06 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1080/19396368.2019.1589601$2DOI 100 1 $aOLIVEIRA, C. S. 245 $aIndividual assessment of bovine embryo development using a homemade chamber reveals kinetic patterns of success and failure to reach blastocyst stage.$h[electronic resource] 260 $c2019 520 $aMost early developmental data are lost in bovine embryo culture systems. We developed and validated a method for culture of bovine embryos in groups that allow individual assessment. An autoclavable low-cost multiembryo chamber (MEC) was prepared using a polyester mesh fixed to a glass coverslip. Embryonic development was not affected by MEC. Compared to conventional bovine culture system (oil-covered drops, control), cleavage (C, 71.2 ± 7.8%; MEC, 74.3 ± 6.0%), blastocyst rate (C, 29.9 ± 4.4%; MEC, 28.3 ± 5.0%) and blastocyst cell number (C, 94.1 ± 9.7; MEC, 92.9 ± 5.3) were similar. Caspase 3 positive cell index in blastocysts was increased in MEC group, but apoptosis rate was below 5% (C, 2.9 ± 0.5; MEC, 4.6 ± 0.6). Using MEC, we performed a retrospective analysis for 'failure' and 'success' embryos, based on their ability to reach the blastocyst stage. We detected the majority of 'success' embryos displayed 8 cells at 48 h post-insemination (hpi) (48.7%), but blastocysts derived from this pattern presented lower cell numbers (91.3 ± 4.2 vs. 107.9 ± 4.9) and higher apoptosis index (6.2 ± 0.6 vs. 4.4 ± 0.5) than blastocysts from 4-cell embryos at 48 hpi. Most (72.0%) embryos that were at morula stage 120 hpi reached blastocyst stage at 168 hpi. Those blastocysts presented more number of cells than blastocysts derived from embryos exhibiting 16 cells at 120 hpi (108.6 ± 4.1 vs. 83.9 ± 4.8). Combination of embryo kinetics data at 48 and 120 hpi revealed high chances of blastocyst formation for patterns: 8 cells/morula, 4 cells/morula, 8 cells/16 cells and 4 cells/16 cells. Blastocysts formed from 4-cell/morula and 8-cell/morula patterns represented 69% of all 168 hpi blastocysts. Blastocysts derived from 4 cells/16 cells displayed decreased apoptosis (3.1 ± 0.6). Our results suggest that MEC can be used for bovine embryo culture without detrimental effects on development and can help to predict blastocyst formation and quality of in vitro fertilization (IVF) embryos. Abbreviations: BSA: bovine serum albumine; COC: cumulus-oocyte complex; FERT-TALP: Tyrode's albumin lactate pyruvate fertilization; FBS: fetal bovine serum; IVF: in vitro fertilization; MEC: multiembryo chamber; PBS: phosphate buffered saline; SOF-AA: synthetic oviductal fluid with amino acids medium; TCM: Tissue Culture Medium. 653 $aCulture system 653 $aEmbryo kinetics 653 $aEmbryonic quality 653 $aSingle embryo culture 700 1 $aBARROS, B. A. F. de 700 1 $aMONTEIRO, C. A. S. 700 1 $aROSA, P. M. S. 700 1 $aLEAL, G. R. 700 1 $aSERAPIÃO, R. V. 700 1 $aCAMARGO, L. S. de A. 773 $tSystems Biology in Reproductive Medicine$gv. 65, n. 4, p. 301-311, 2019.
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